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Gram’s Crystal Violet

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Gram's Crystal Violet is used for Gram's staining. In practice, a smear of bacterial cells is stained with crystal violet, then treated with an iodine containing mordant to increase the binding of primary stain. A decolourizing solution is used to remove the crystal violet from cells which bind it weakly and then the counterstain with safranin.

Gram's Crystal Violet is used for Gram's staining. In practice, a smear of bacterial cells is stained with crystal violet, then treated with an iodine containing mordant to increase the binding of primary stain. A decolourizing solution is used to remove the crystal violet from cells which bind it weakly and then the counterstain with safranin.

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