Fluid Thioglycollate Medium (Thioglycollate Medium, Fluid)
Recommended for rapid cultivation of aerobes as well as anaerobes including microaerophiles by adding a reducing agent and small amount of agar. The USP, BP, EP and AOAC have recommended the media for sterility testing of antibiotics, biologicals and foods and for determining the phenol coefficient and sporicidal effect of disinfectants. it is intended for the examination of clear liquid or water-soluble materials. FTM is also routinely used to check the sterility of stored blood in blood banks.
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EMB Agar, Levine
This medium was developed by Levine used for the differentiation of Escherichia coli and Klebsiella aerogenes and also for the rapid identification of Candida albicans. This medium is recommended for the detection, enumeration and differentiation of members of the coliform group by APHA. Levine EMB Agar, with added Chlortetracycline hydrochloride can be used for the rapid identification of Candida albicans in clinical specimens.
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DNase Test Agar Base
DNase Test Agar Base is recommended for detection of deoxyribounclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci. Addition of toluidine blue in the media is used for differentiation and identification of non-pigmented Serratia species isolated from clinical sources that might be improperly identified as Enterobacter and Klebsiella species. Positive DNase activity is visualized as clear zones (around colonies) when the plates were flooded with 1 N hydrochloric acid.
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Columbia Blood Agar Base
This medium is used as the base for the media containing blood and for selective media formulations in which different combinations of antimicrobial agents are used as additives. It is also selective for the isolation of Haemophilus species from clinical specimens, especially from upper respiratory tract.
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Cetrimide Agar
This granulated media formulation is in accordance with the harmonized method of USP/EP/BP/JP/IP. It is used as a selective medium for the isolation of Pseudomonas aeruginosa from pharmaceutical products. This medium is also used for microbial limit testing for non- sterile products. Ps.aeruginosa colonies may appear pigmented blue, blue-green or non-pigmented. Colonies exhibiting fluorescence at 250nm and a blue green pigmentation are considered as presumptive positive.
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C.L.E.D. Agar w/ Bromo Thymol Blue
Description
C.L.E.D. Agar w/Bromo Thymol Blue is recommended for use in urinary bacteriology. This medium promotes the growth of all urinary pathogens. Lactose is the fermentable sugar. Bromo thymol blue is the pH indicator which turns yellow at acidic pH. Colonies grow as blue or yellow due to fermentation of lactose.
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Bile Esculin Agar
Bile Esculin Agar is recommended for isolation and presumptive identification of group D Streptococci from food and pharmaceutical products. Enterococci and Group D Streptococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing brownish black precipitate. Identification of enterococci and differentiation of Group D strptococci from non Group D can thus be performed using this agar medium.
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Baird Parker Agar Base
Recommended for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase positive strains. the use of Baird-Parker Agar was officially adopted by AOAC International and is recommended in the USP for use in the performance of Microbial Limit Tests. Recently, ISO committee has also recommended this medium for the isolation and enumeration of Staphylococci. The isolation and enumeration of coagulase positive Staphylococci from food and clinical samples
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Agar Agar, Type I
Agar Agar, Type I is recommended for preperation of culture media for microbiological analysis.
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*Salmonella Differential Agar (Twin pack) (RajHans Medium)
Description
used for differentiation of Salmonella species from Proteus species and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella species and is utilized in these media
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*Sabouraud Chloramphenicol Agar
This medium is recommended for cultivation of yeasts and moulds. This medium was described originally by Sabouraud for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is often used with antibiotics such as Chloramphenicol for the isolation of pathogenic fungi from materials containing large numbers of fungi or bacteria.
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