This media also known as RMW Agar is used for isolation, enumeration and identification of Lactobacilli from foodstuffs and clinical specimens. Low pH of the medium and addition of acetic acid makes the medium selective for Lactobacilli inhibiting other bacterial flora. It is recommended that the plates should be incubated at 30°C for 5 days or at 37°C for 3 days in an atmosphere of 95% hydrogen and 5% carbon dioxide. If this is not possible, overlay the inoculated plates with a second layer of the agar before incubation. All colonies should be checked by gram staining and by catalase test before further identification. The salt in the formulation makes the medium unsuitable for isolation of dairy Lactobacilli. e.g. L. lactis, L. bulgaricus and L. helveticus.

PPotassium hydroxide (KOH) is a hygroscopic, water soluble base synthesized by the electrolysis of potassium chloride solution. It is the raw material for the synthesis of many potassium compounds.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Piperacillin / Tazobactam is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with PIT and concentration 30/6 mcg for easy identification.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Meropenem is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with MRP and concentration 10 mcg for easy identification.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Imipenem is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with IPM and concentration 10mcg for easy identification.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Fosfomycin is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with FO and concentration 200 mcg for easy identification.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Cefoxitin-Cloxacillin is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with CXX and concentration 30/200 mcg for easy identification.

Antibiotic Sensitivity disc are used to determine the effectivness of an antibiotic against a microorganism as a guide to antibiotic therapy. The disc consist of a specific concentration of an antibiotic in mcg which is tested against a microorganism of a standard density, swabbed uniformly on an appropriate media following overnight incubation. Cefotaxime/Clavulanic acid is impregnated on a filter paper disc of specified concentration. Both sides of the disc are printed with CEC and concentration 30/10 mcg for easy identification.

Benedict's reagent is used to test for the presence of reducing sugars. Benedict’s quantitative reagent is a modification of qualitative aspects. It contains copper sulphate-sodium carbonate. It also contains potassium thiocyanate and small amount of potassium ferricyanate.The reduction of Cu3+ ions by sugar is a non-stoichiometric equation and is only constant over a small range of sugar concentration.

Oxidase Discs are used for detection of oxidase production by microorganisms like Neisseria, Alcaligenes, Aeromonas, Vibrio's, Campylobacter and Pseudomonas, which give positive reactions and for excluding Enterobacteriaceae, which give negative reactions. Oxidase discs are sterile filter paper discs impregnated with N, N-dimethyl-p-phenylenediamine oxalate, ascorbic acid and a-naphthol. These discs overcome the neccessity of daily preparation of fresh reagent. Pseudomonas aeruginosa ATCC 27853 positive : deep purplish blue colouration of disc ; Staphylococcus aureus ATCC 25923 negative : no colour change.